Target Molecular Size of the Red Beet Plasma Membrane
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چکیده
Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using -y-ray radiation from a '37Cs source. Inactivation of vanadate-sensitive ATPase activity by y-ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit of the red beet plasma membrane ATPase has been demonstrated to have a molecular weight of about 100,000 by dodecyl-sulfate gel electrophoresis following 32P-phosphorylation, it is suggested that the native enzyme may exist, at least, as a dimer of
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Target molecular size of the red beet plasma membrane ATPase.
Radiation inactivation of the red beet (Beta vulgaris L.) plasma membrane ATPase was carried out using gamma-ray radiation from a (137)Cs source. Inactivation of vanadate-sensitive ATPase activity by gamma-ray radiation followed an exponential decline with increasing total dose, indicating a single target size calculated to have a molecular weight of about 228,000. Since the catalytic subunit o...
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